New Insights Into Cleft Palate Unlocked With 10x Genomics' Single Cell and Spatial Technologies
Researchers used Chromium Single Cell products and Xenium In Situ to profile how gene expression is spatially regulated during secondary palate formation
Annually, clefts of the lip and/or palate occur in ~1 in every 700 live births. Despite its prevalence, there is still a very limited understanding of how the bones forming these foundational facial structures develop and pattern as an embryo develops. In this study, "Spatial Multi-omics Reveals the Role of the Wnt Modulator,
The study's first author,
"Xenium In Situ's workflow and analysis pipeline allowed us to optimize the assay conditions for our target tissue, run the whole protocol and analyze all replicates in-house in less than one month. The intuitive design of the bench workflow and post-run analysis software may be the most valuable aspects of this high-throughput in situ technology to accelerate science."
As part of their initial assessment, the researchers performed an integrated assessment of the transcriptomic and epigenomic profiles of wild-type and Pax9-deficient mice using the Chromium Single Cell Multiome ATAC + Gene Expression (Multiome) assay. Paired with 3D-micro-computerized tomography (micro-CT) bone imaging analysis showing disrupted patterning of the palate in Pax9-deficient mice, the authors hypothesized that loss of Pax9 expression disrupts Wnt signaling dynamics in a way that influences the process of proper bone formation.
To further investigate the relationship between Pax9 and Wnt signaling, the researchers created a fully custom gene expression panel to profile 350 genes at single cell spatial resolution with the Xenium In Situ platform. The researchers customized their gene panel to focus on cell-type specific markers, genes involved in signaling interactions and other genes of interest uncovered in the Multiome data.
The Xenium analysis revealed significant spatial gene expression differences between wild-type and Pax9-deficient mice, indicating a role for Pax9 in regulating the differentiation and maturation of a specific subset of progenitor cells. Of particular note, Xenium revealed that disrupting Wnt signals blocks the extension of the palate to the midline in this cleft palate model, a process that could be targeted for discovery of potential in utero and early postnatal treatments to correct cleft palate anomalies.
To learn more about this study, read the full article.
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10x Genomics is a life science technology company building products to accelerate the mastery of biology and advance human health. Our integrated solutions include instruments, consumables and software for single cell and spatial biology, which help academic and translational researchers and biopharmaceutical companies understand biological systems at a resolution and scale that matches the complexity of biology. Our products are behind breakthroughs in oncology, immunology, neuroscience and more, fueling powerful discoveries that are transforming the world's understanding of health and disease. To learn more, visit 10xgenomics.com or connect with us on LinkedIn or X (Twitter).
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